The QIAamp DNA Microbiome Kit uses the same proven spin column technology of other QIAamp kits with a modified protocol designed to evenly enrich the bacterial microbiome DNA content of a sample. RNA binds to the membrane and contaminants are washed away. The quality of RNA purified using the QIAcube is comparable to the manual procedure (see figure Comparison of automated and manual RNA purification). Both DNase digestion on the RNeasy 96 plate as well as the gDNA Eliminator 96 plate with RNeasy Plus 96, eliminate DNA contamination with similar efficiency. Vacuum manifold for processing 124 spin columns: includes QIAvac 24 Plus Vacuum Manifold, Luer Plugs, Quick Couplings. For vacuum processing of QIAGEN spin columns, For QIAvac 24, QIAvac 6S, QIAvac 96, Vacuum Regulator. The exact composition of Buffer RLT is confidential. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 l, with a preparation time of 2040 minutes. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? A convenient tool to build experimental workflows and find products to match your needs. The QIAcube is highly suitable for academic research laboratories as well as pharmaceutical, biotechnology, and biomedical research laboratories performing applications, such as: For an up-to-date list of available protocols, visit QIAcube Standard Protocols. Purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit, Selection guide for RNA isolation for all sample types, Acetone precipitation of protein from Buffer RLT or Buffer RLT Plus lysates, Effects of malnutrition on expression of lactase in children, Efficient gDNA removal with unique gDNA Eliminator columns or plates (no need for DNase), High-quality total RNA in minutes using fast and simple extraction protocols, High-throughput processing in 96-well format, Ideal for sensitive applications such as real-time RT-PCR and RNA-seq, prevent overloading by adjusting the amount of starting material tono more than the maximum amounts recommendedin the, strictly follow the protocol for on-column DNase Digestion in Appendix D of the RNeasy Mini Handbook (you can let wash buffer RW1 incubate on the column for 3-5 minutesbefore centrifuging to enhance removal of excess gDNA prior to applying the enzyme). Find the right products for every step of your experiment effortlessly. Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? Explore our new, easy-to-navigate digital Product Profile! The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Sample purification can be performed manually, or can be automated using the QIAcube, QIAsymphony SP, QIAxtractor, EZ1 Advanced, EZ1 Advanced XL, or BioRobot, or BioSprint instruments. The labware document will list the sample tubes that have been tested for your application. Different protocols are available for different starting materials. What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen RNA Quantitation Reagent for RNA, and PicoGreen DNA Quantitation Reagent for DNA (Molecular Probes, Inc.). The RNeasy Plus 96 Kit allows extraction of RNA from up to 1 x 10e6 cells with the vacuum/spin protocol, or up to 2 x 10e6 cells with the spin protocol. When should you use targeted DNA sequencing? A convenient tool to build experimental workflows and find products to match your needs. The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking. RNA purification using RNeasy 96 plates is manual, and comprises 3 simple steps: bind, wash, and elute. A convenient tool to build experimental workflows and find products to match your needs. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets. For simultaneous purification ofDNA, RNA, and protein fromthe samesample (either cultured cells or easy-to-lyse tissues), we recommend using the AllPrep DNA/RNA/Protein Mini Kit. 20, QIAcube Software, Mainboard Firmware/Mainboard PLC Software/Centrifuge Firmware P/S/H (Windows platforms) (EN), QIAcube Software, Mainboard Firmware/Mainboard PLC Software/Centrifuge Firmware P/S/H (other platforms) (EN), (EN) - Automated DNA purification from diverse microbiome samples using dedicated microbiome kits on the QIAcube, QIAcube RNA isolation from stool samples using the RNeasy PowerMicrobiome Kit, Sequential automation of RNA and DNA preps on the same QIAcube instrument, (EN) - Automated extraction of forensic samples using established spin column technology on the QIAcube, (EN) - Workflow for DNA purification from tough specimens, (EN) - QIAcube Tip-Adapter Ring Replacement, https://www.qiagen.com/qiacube/customized/protocoladd.aspx, http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx, http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation, 12,000 x g maximum, swing-out rotor with 12 positions, maximum 45, Relative humidity of 10-75% (noncondensing), Protocols/main application on this instrument, DNA purification, RNA purification, protein purification, 100-120 V AC, 50-60 Hz, 650 VA (North America and Japan), 220-240 V AC, 50-60 Hz, 650 VA (Europe), mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages, Most QIAGEN manual kits (e.g., QIAamp, QIAquick, QIAprep), Speed 100-2000 rpm, amplitude 2 mm, heating range of room temperature to 70C, ramp-up time of <5 min from room temperature to 55C (+/-)3C), Syringe size 1 ml, pipetting range 5-900 l, QIAGEN protocols are preinstalled on the QIAcube or can be downloaded at www.qiagen.com/MyQIAcube, Transmissive TFT, 64 x 86 x 65 mm, white LED backlight, high brightness, Bisulfite converted DNA derived from FFPE tissues, 10-16 l in increments of 1 l; default 16 l, Bisulfite converted DNA, Bisulfite converted DNA derived from FFPE tissues, 30-100 l in increments of 10 l, default 50l, Automation of trusted QIAGEN spin-column kits, More free time with affordable automated processing, Standardized results and increased productivity, Mikrozid Liquid (Schlke & Mayr GmbH) ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid), RBS-35 Detergent Concentrate (Pierce Chemical Co.) anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door, RNaseZap (Ambion) for complete removal of RNase contamination from glass and plastic surfaces, 0.1 M NaOH for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap). Short-term storage (up to 4 weeks) at room temperature (1525C) does not affect the performance. Cell-Free DNA; DNA Clean Up; Genomic DNA; Microbial DNA; QIAamp Viral RNA Mini Handbook. See:http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation for a virtual demonstration on how to use the QIAcube. 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? For this, just connect your QIAcube Connect to QIAsphere. Size of ribosomal RNAs from various sources. A convenient tool to build experimental workflows and find products to match your needs. What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? Primers, nucleotides, enzymes, and other impurities are efficiently removed and recovery of DNA is comparable to the manual procedure (see figure High recovery of PCR products). the tissue samples can be positioned more easily in the microtome and have better qualities during sectioning. In case DNA as well as RNA from precious samples is of interest from the identical sections, please have a look at the AllPrep DNA/RNA Kits for unfixed tissue or the AllPrep DNA/RNA FFPE Kit for fixed tissue. DNA yields comparable to the manual procedure. In general, disruption works very well using one stainless steel bead with a diameter of 5 mm. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. 7.35 kg (net); approx. The purified DNA is free of proteins, nucleases, and other inhibitors. No. 79216). See: TissueLyser Virtual Demofor a demonstration on how these beads are used with the TissueLyser LT. Yes. The RNeasy Plus Micro Kit is specially designed for limited amounts of samples and isolates up to 45 g pure total RNA The RNeasy Plus Mini Kit purifies up to 100 g total RNA (>200 nt) from a single extraction with efficient gDNA removal. Ethanol is added to provide appropriate binding conditions for RNA, and the sample is applied to an RNeasy spin column or 96-well plate. What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? The crosslinking causes the RNA to break, resulting in overall smaller molecules, which look like a smear when analyzed on a formaldehyde gel. However, for optimal performance and quality, storage temperature should not exceed 25C. For instructions on how to operate the centrifuge, refer to the QIAcube Connect User Manual, chapter 5 "Operating Procedures",section 5.8 "Operating the Centrifuge". The TRACKMAN Connected system guides researchers through the RNeasy Plus Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. The QIAcube has the following weight and dimensions: Please navigate to the Product Page of the QIAsymphony Kit you are using; for example, the QIAsymphony RNA Kit, Cat No./ID: 931636. No. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type. I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 28C upon receipt. Yes, RNAs smaller than 200 nucleotides, including miRNA, can be isolated using the AllPrep DNA/RNA Micro or RNeasy Plus Micro Kit. What sample types can be used with the AllPrep DNA/RNA 96 Kit? The TissueLyser LT works in combination with the TissueLyser LT Adapter. This is a collection of all cleanup protocols for the QIAcube classic. In our labs, we were able to purify RNA using the RNeasy Plus MicroKit,or DNA/RNA using the AllPrep DNA/RNA Micro Kitatsingle cell level. For more information please seethe TissueLyser LT Handbook. QIAamp DNA Mini and Blood Mini Kit. See: TissueLyser Virtual Demofor a virtual demonstration on how the TissueLyser LT Adapter is used. Percentage recovery was determined spectrophotometrically. No. Filter-Tips, 1000 l wide-bore (1024), cat no. Download Safety Data Sheets for QIAGEN product components. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. Possible candidates that can increase the A230 include salt, carbohydrates, peptides, and phenol (or aromatic compounds in general). To overcome this, QIAGEN columns are inserted into disposable VacConnectors, preventing direct contact of the column with the manifold. Can I still use them? For more information about the different applications please refer to the. 990352. If disrupting fresh or frozen samples, the TissueLyser LT Adapter can be precooled on dry ice to prevent degradation of nucleic acids and proteins (see figures "High-quality DNA from plant tissues" and "Intact RNA from plant tissues"). The QIAcube Connect centrifuge can be operatedindependently if the QIAcube Connect is not performing a protocol run. A convenient tool to build experimental workflows and find products to match your needs. How can I decontaminate my QIAsymphony SP / AS system? 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? This product is not intended for the diagnosis, prevention, or treatment of a disease. When disrupting and homogenizing tissues in Buffer RLT Plus (supplied with the RNeasy Plus Mini Kit), excessive foaming may occur. Instructionsare presentedin Appendix C of the RNeasy MinElute Cleanup Handbook. See: TissueLyser Virtual Demofor a virtual demonstration of this process. The last protocol step performed by the QIAcubewill bedisplayed in the touch screen. If the distance of the "receiving" plate to the nozzles is not optimal, splashing and thus cross contamination can occur. Theconvenient spin-column procedure reduces hands-on preparation time to 20 minutes. However,using the gDNA Eliminator 96 plate of the RNeasy Plus 96 Kitis faster and more economical. We do not sell the gDNA eliminator plates separately. The QIAamp DNA Blood Mini Kit procedure is Pure RNA should yield an A260/A230 ratio of around 2 or slightly above; however, there is no consensus on the acceptable lower limit of this ratio. The QIAcube together with the QIAprep Spin Miniprep Kit enables fully automated purification of up to 20 g molecular biology grade plasmid DNA. Purified DNA is suitable for use in routine molecular biology applications, such as fluorescent sequencing, cloning, or transfection of robust cells (see figure Reliable sequencing results). What is the cellular composition of human blood? The TissueLyser LT provides effective disruption of human, animal, and plant tissues, bacteria, and yeast, allowing reproducible yields of DNA, RNA, and protein in subsequent purification procedures (see figure "Effective tissue disruption"). If processing either 1 sample or 11 samples, load an additional, empty sample tube to balance the TissueLyser LT Adapter. What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield? A custom protocol can be requested through the online QIAcube protocol request page: <. What is the concentration of the resuspended Protease? The lysate is then passed through a gDNA Eliminator spin column or gDNA Eliminator 96 plate that, in combination with the high-salt buffer, selectively and efficiently removes genomic DNA. Disruption with the TissueLyser LT is comparable to that achieved with the well-established TissueLyser II (see figures"Effective tissue disruption", "Intact protein suitable for all types of analysis", "High-quality DNA from animal tissues", and "High-quality DNA from plant tissues"). Highly reproducible protein purification. Short-term storage (up to 4 weeks) at room temperature (1525C) does not affect the performance. How can I decontaminate the QIAcube system? Try the Workflow Configurator. How can I precipitate genomic DNA using isopropanol? How can I check the integrity of RNA purified using RNeasy Kits? Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? These procedures have been used successfully for isolation of genomic DNA from. DNA and RNA purified using the AllPrep DNA/RNA FFPE Kit are of comparable quality to DNA and RNA purified using the QIAamp FFPE Tissue Kit and RNeasy FFPE Kit/miRNeasy FFPE, respectively (see figure " Purification of DNA and RNA from FFPE samples ").The purified nucleic acids are therefore suitable for downstream applications such as Pyrosequencing or real-time Purification of DNA using the QIAamp DNA Investigator Kit can be automated on the QIAcube Connect. The innovative QIAcube controls integrated components including a centrifuge, heated shaker, pipetting system, and robotic gripper. The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. What are the dimensions of the TissueLyser LT? The amount of starting material is dependent on the sample material that should be disrupted. Rotor-adapters supplied with the kits are preloaded with spin columns and elution tubes, delivering greater convenience and time savings. Are run reports and/or log files available on the QIAcube? What is Tissue-Tek O.C.T., and what is it used for? RNeasy Plus Mini standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. No, we have never observed coamplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptomeprotocol when using RNA purified with RNeasy Kits without on-column DNase digestion. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies all in GeneGlobe. The two protocols given below are for the use of the Ni-NTA Superflow 96 BioRobot Kit in manual procedures. To improve digestion of tough tissue samples, Proteinase K incubation at 56C can be performed overnight. This site is protected by reCAPTCHA and the Google, "High, reproducible yields of genomic DNA", "Vacuum-driven liquid processing on vacuum manifolds", "An integrated system for various applications". See: TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT works. Coolable adapter to prevent biomolecule degradation. Simply place a QIAGEN 96-well purification plate in the QIAvac 96 top plate, making sure that the plate is seated tightly and turn on the vacuum source. Cooling the adapter and sample tubes on dry ice or in a freezer to approx. For optimal results, we recommend using the QIAvac 24 Plus and QIAvac 96 together with the QIAvac Connecting System and a Vacuum Pump. A maximum of 5 l RNA eluate from RNeasy extraction procedures can be added to the reverse-transcription reaction with the QuantiTect Whole Transcriptome Kit. Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? Short-term storage (up to 4 weeks) at room temperature (1525C) does not affect the performance. Product Profile - QIAamp genomic DNA kits, Purification of total RNA from tissues using the RNeasy Plus 96 Kit and vacuum/spin technology - (EN), Purification of genomic DNA from the PAXgene Blood ccfDNA Tube using the QIAamp DNA Blood Mini Kit - (EN), Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Blood Mini Kit; vacuum procedure - (EN), Manual purification of 6xHis-tagged proteins from E. coli using the Ni-NTA Superflow 96 BioRobot Kit - (EN), Isolation of plasmid DNA from Agrobacterium using the QIAprep Spin Miniprep Kit; vacuum procedure - (EN), Purification of viral RNA and DNA from 1000 l of plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit - (EN), Reduces sample handling and accelerates processing. Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? For more information on efficient sample disruption and homogenization for nucleic acid extractions, please see further product details and resources for theTissueRuptor, TissueLyser LT, and TissueLyser II. For information on how to remove these parts, refer tothe QIAcube User Manual. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies all in GeneGlobe. The QIAamp DNA Investigator Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 l. This product is not intended for diagnosis, prevention, or treatment of a disease. See: TissueLyser Virtual Demofor a demonstration of cooling the samples on dry ice before using the TissueLyser LT. Do you have a protocol for the detection of Bordetella pertussis DNA by PCR? Want to quantify 16 nucleic acid samples in under 2 minutes? The protocols differ mainly in the lysis and homogenization of the sample. DNA yields were determined using a spectrophotometer. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies all in GeneGlobe. Most cryosections are fixed using non-crosslinking agents. What applications are offered on the QIAcube? What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit? RNA purified using RNeasy Plus Kits is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as RNA-seq and quantitative real-time RT-PCR. The exact composition of Buffer RW1 is confidential. no 990394, and the required disposable tips: need to be ordered separately. Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings? Looking for a quick way to design experiments? [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. Yields of nucleic acids or DNA depend on the starting material (see table). If the ribosomal bands are not sharp, but appear as a smear of smaller sized RNAs, it is likely that the RNA sample has suffered major degradation during preparation. The procedure provides an enrichment for mRNA since most RNAs <200 nucleotides (such as rRNAs and tRNAs) are excluded. These products are not intended for the diagnosis, prevention, or treatment of a disease. Note: If a protocol run is stopped, the run cannot be restarted. The QIAcube and Ni-NTA Spin Kit provide reliable purification of highly pure 6xHis-tagged proteins (see figure Highly reproducible protein purification). Do you have a protocol for the isolation of genomic DNA from sperm? Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit? Tissues stored in Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein) or in RNAprotect Tissue Reagent (to stabilize RNA) require no precooling of the adapter. This protocol has only been tested with soft tissues (e.g., liver, spleen, thymus, heart, kidney, and brain) and may not work with hard tissues (e.g., bone, teeth, and skin). How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate? What is the typical amount of tissue used for disruption with the TissueLyser LT? The QIAsymphony system has an integrated UV lamp. Find the right products for every step of your experiment effortlessly. Using QIAamp MinElute spin columns for purification of high-quality quality DNA with flexible elution volumes, the QIAamp DNA Investigator procedure consists of 4 steps (see flowchart ", Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit, Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL.