Oil pollution may arise either accidentally or operationally whenever oil is Four major cities in Nigeria, Onitsha, Kaduna, Aba and Umuahia, were recently listed by the World Health Organisation (WHO) as some of the most polluted places in the world. According to the study, however, bioremediation techniques ranging from non-supplemented in-situ and fertilizer supplemented in-situ to mixed in-situ and ex-situ bio-cells supplemented RENA are feasible approaches for spill sites. Biostimulation of the activity of degrading autochthonous microbiota constitutes one of the most accepted bioremediation tools for oil-polluted soils. Continue Reading. A microbiological study of the self-cleaning potential of oily Arabian Gulf coasts. Bacteria are the most common bioaugmentation organisms. In some studies, enhanced degradation with immobilized cells as compared to free living bacterial cells for the treatment 2006; 57:222-228. doi: 10.1016/j.ibiod . Antonie Van Leeuwenhoek. The immobilization of whole cells. Mineral salt medium to which crude oil had been added was used as a sole source of carbon and energy to isolate hydrocarbon utilisers from soil samples collected from different plots of the contaminated site. These matrixes have porous structure, and thus the pollutant and various metabolic products could easily diffuse through into the matrix. Use of microorganism-immobilized polyurethane foams to absorb and degrade oil on water surface. AIMS Microbiol. 2014;82(1-2):3944. Applications of nutrients such as nitrogen fertilizers, oleophilic fertilizers or surfactants are some of the strategies employed. The white rot fungus, Pleurotus tuberregium, was examined for its ability to ameliorate crude oil polluted soil. and can be occurred for a longer period. Knowledge of soil parameters such as caution exchange capacity, relevant nutrient availability, acidity (soil pH), aeration or oxygen level, hydraulic properties etc are paramount and this requires the assistance of specialists in these areas. Immobi-lization can be occurred for enzymes, cellular organelles, animal and plant cells. Alginates (polymers made of different proportions and sequences of mannuronic and guluronic acids extracted from brown algae) are the polymers of choice in most systems of immobilization because they are easy to handle, nontoxic to humans, the environment, and the entrapped microorga-nisms, legally safe for human use, available in large quantities and inexpensive. Hassanshahian M., Zeynalipour M.S., Musa F.H. Xu and Lu [44] demonstrated that oil removal in a crude oil-contaminated soil was increased by application of hydrocarbon-degrading bacteria immobilized on peanut hull powder as biocarrier. Soil pH, electrical conductivity, and phytotoxicity to seed germination and growth of maize (Zea mays) served as risk assessments on soil quality and evidence of recovery for the oil-impacted soil. A kinetic study on the bioremediation of sodium cyanide and acetonitrile by free and immobilized cells of, White G.F., Thomas O.R. Easy separation of cells and carrier from media. The most important requirement is that bacteria with appropriate metabolic capabilities must be present. From the results obtained it can be concluded that cow dung has demonstrated high potential in enhancing bioremediation of crude oil contaminated soil. Composite samples, collected under appropriate conditions and methods (before and after treatment) were sent to an ISO certified laboratory in the United States of America, USA (by courier) and another in Nigeria for the analyses. Information was also obtained from the staff of the oil company on the mode of RENA remediation works carried out at the study site. In the interaction between microorganism and the surface of the matrix, weak forces are involved include hydrogen bonds, ionic bonds, hydrophobicbonds and van der Waals forces. Bioremediation of crude oil polluted seawater by a hydrocarbondegrading bacterial strain immobilized on chitin and chitosan flakes. Positive correlations were obtained for plant agronomical parameters and growth period, for all treatments, with coefficients in the range of .905 to .996, p < .05. Bioremediation of crude oil using immobilized cells is rarely studied. Bioremediation of oil-contaminated seawater and sediment by an oil-degrading bacterial Consortium. Crude oil is one of the essential compounds in both the energy and chemical industries (Li et al., 2016).However, crude oil leakage caused by natural or human factors results in serious environmental pollution and sustainability problems worldwide (Gu and Wang, 2015; Muthukumar et al., 2022).Most of the components in crude oil can be stored and bioaccumulated in animals and . Artificially polluted soil with %1density of crude oil was used and then fertilizers were applied in 3 levels of 0, 1 and 2 ton/ha in 3 replicates. 8600 Rockville Pike The increasing of environmental pollution and the treating contaminated sites are necessary atat present [35]. The major remediation technique adopted by one of Nigerias leading international oil companies (the Shell Petroleum Development Company, Port Harcourt, Nigeria) for crude-oil-impacted soil, at the time of study, is referred to as RENA (Remediation by Enhanced Natural Attenuation). Degradation of naphthalene by cells of. The RESULTS suggested that polyurethane was a better immobilization matrix than calcium alginate owing to its greater mechanical strength and improved oxygen transfer characteristics. official website and that any information you provide is encrypted Preparation and industrial application of immobilized aminoacylase. Providing suitable micro environmental conditions. [43] used immobilized the bacterial consortium MPD-M on polypropylene fibers for biodegradation of crude oil in water with salinities varying from 0 to 180 g L-1. This study aims to investigate bacteria for biodegradation of oil pollutants from oily industrial wastewater to be used as bioremediation tools and to determine the characterization of . potential approaches for bioremediation such as risk-based remediation, and regulatory assessment procedures for The academic, renowned for his advocacy in the field of learning, emerged the winner in the education category in a ceremony that produced 41 other winners in Abuja on Saturday. [53] investigated the degradation of p-cresol by a Pseudomonas sp. Hazard. 2015; 3(3):79-85. doi: 10.12691/ijebb-3-3-2. Transformation of 2,4,6-trinitrotoluene (TNT) by immobilized and resting cells of. Assessment Of Crude Oil Pollution And Its Effect On Particle Sizes On Bioremediation. They can be classified to three physiological states consist of dead, living and growing states. The last stage of any bioremediation project should include bioassay of the treated site. Hwang et al. The use of biological remediation has helped to detoxify and restore the ecosystems damaged by crude oil spillage. Immobilization hydrocarbons degrader's bacteria have high potential to clean up oil contamination and can be facilitating oil biodegradation in polluted environment. IN SITU TREATMENT OF SOIL AND GROUND-WATER CONTAMINATED . Soil contamination by heavy metals is a worldwide problem for human health and safe food . [ad] Hassanshahian M., Emtiazi G., Caruso G., Cappello S. Bioremediation (bioaugmentation/biostimulation) trials of oil polluted seawater: a mesocosm simulation study. It is one of the most dangerous pollution factors known today. The authors tested the ability to survive and degrade hydrocarbons for 6 weeks at 45 C. the Environment Microorganisms and. Leon R., Fernandes P., Pinheiro H.M., et al. 2014;95:2838. Bioremediation is a process that degrades environmental pollution by microorga-nisms [4, 5]. This process is known as bioremediation in general and is a procedure authorized by the EPA to reverse the existence of oil or gas spills [11]. HHS Vulnerability Disclosure, Help In this study, we aim to investigate the efficiency of crude oil bioremediation through composting and culture-assisted composting. Biostimulation options were (i) introduction of mineral fertilizers and (ii) periodic application of different amounts of water. Please enable it to take advantage of the complete set of features! The contamination of soils has resulted in severe environmental deterioration (Su et al. 2 depicts microalgae bio fuel production. The use of polyurethane foams developed due to wide range of porosity, mechanical properties, hydrophobicity and hidrophilicity of polyurethane foams. Therefore some basic steps that may be necessary for a successful bioremediation project will include compliance analysis, site characterization, method selection / feasibility studies, remediation proper and end for project analysis (Bonaventura, et al., 1995). Degradation of pentachlorophenol by polyurethane-immobilized. 2.0 Bioremediation. Persistent Organic Pollutants POPs Environmental Risks. Many of these compounds are naturally transformed to metabolites of unknown persistence and toxicity. Send the Following Details on WhatsApp ( 08143831497) After Payment. Bookshelf -, Chemosphere. The selection of carrier is very important for use in immobilization. isolated from fermented cassava steep can be used for bioremediation of oil polluted stagnant waters. Petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. This site needs JavaScript to work properly. Received 2014 Dec 1; Revised 2015 Apr 13; Accepted 2015 Apr 14. They are not needful tThe processes for the extraction, separation and purification of the enzymes from the cells are not necessary. The harm that oil pollution causes to the ecological environment is well known (Sikkema et al., 1995).For example, the Deep Water Horizon oil spill accident in the Gulf of Mexico produced a profound impact on the economy and environmental safety, which is still the focus of people's attention (Xue et al., 2015). The medium is not excavated and transported to another loca-tion for treatment. Application, bacteria, biodegradation, crude oil, soil. Recently, Maliji et al. Percentage germination, seedling height, seedling girth, number of leaves and number of nodules decreased as the concentrations of crude oil in soil samples increased. This study revealed that ready-to-use composted waste has the potential for bioremediation of soils polluted with petroleum and petroleum products. In a situation where, for instance, the end-use of the land is farming, using reduction of petroleum hydrocarbon concentrations as the only or major index for closeout of remediation projects without recourse to other ecological and socio-environmental factors poses some threats to the environment in terms of soil quality, food security, food safety and means of livelihood for the populace. Accessibility The high immobilization efficiency of the cells onto the immobilization material and the high affinity between the hydrophobic immobilization material and the substrates caused excellent degradation. Organic carrier can be divided into natural and synthetic polymers. 3 Biotech. Epub 2015 Oct 19. In some cases, microbial metabolism of petroleum hydrocarbons may produce toxic metabolites such as naphthenic acids, which can hamper subsequent biodegradation due to their toxicity that represses microbial metabolism [51]. Blood Urine Barrier -glomerular filtration barrier. Limitations of in situ biodegradation via land farming where environmental controls are not put in place are highlighted. sharing sensitive information, make sure youre on a federal Macmillan Publishing Co., New York, In: Encyclopedia of Environmental . They observed the immobilized cells significantly increased the biodegradation rate of crude oil compared with free-living cells, the bacterial consortium MPD-M was highly stable in immobilized systems and it was not greatly affected by addition in salinity, also the biodegradation of pristanepristine (PR) and phytane (PH) and of the aromatic fraction was also increased using cells immobilized on polypropylene fibers. The essence of bioremediation is to reinstate and naturalize the polluted environment to ensure it pleasantness (Hatzikioseyian 2010; Enerijiofi 2021). sand used in methanogenic fluidized beds), the inorganic supports also lendthemselves more conveniently to scale-up [22, 23]. Learn more Another study examined the impacts of selected leguminous tree species and kaolinite pre-amendment on oil-contaminated soil for bioremediation in the oil-bearing region of Nigeria. Bioremediation of diesel-contaminated soil by microorganisms immobilized in polyvinylalcohol. Several strains of bacteria, fungi, algae and plants are capable of breaking down harmful substances to nontoxic form. It has been the consensus of stakeholders that to promote the protection of the natural world, continuous intellectual research and campaign on sustainable environment is a must. Oil spills are a major menace to the environment as [49] used natural support such as luffa and sponge for immobilization of Bacillus cereus in diesel Oil degradation. Application of bioemulsifiers in soil oil bioremediation processes. Although the promise of bioremediation is yet to be realized, innovative areas in Environmental Biotechnology for oil spill cleanup are highlighted. Bioremediation of oily sea water by bacteria immobilized in biofilms coating microalgae. Between 38 to 57% degradation of crude oils (with densities ranging from 25,800 to 77,200 mg/kg dry weight) in contaminated soils was achieved after 53 days of operation. Tariq A., Latif Z. Bioremediation of mercury compounds by using immobilized nitrogen fixing bacteria. Environmental pollution has been rising in the past few decades due to increased anthropogenic activities. Biopesticides are also explained as an alternative to conventional pesticides. Abstract Crude oil contaminated soil from Isaka mangrove in Okirika local government area of Rivers state was treated with three different organic wastes (goat manure, poultry droppings and cow dung), for a period of 28 days. An official website of the United States government. All the samples, including the controls, were closely observed for a period of seven weeks at one-week interval for the physiochemical parameters such as pH, Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD), Total Hydrocarbon Content (THC), turbidity, and total microbial count. There are many types of support and immobilization techniques that can be selected based on the sort of application. Al-Kaabi N, Disi ZA, Al-Ghouti MA, Solling TI, Zouari N. Biotechnol Rep (Amst). Researchers have demonstrated the efficacy of the novel, eco-safe and nanotechnology based bioremediation technique (CNB-Tech) in the remediation and restoration of petroleum impacted soils to beneficial end products. Wilson N.G., Bradley G. Enhanced degradation of petroleum (slovene diesel) in an aqueous system by immobilized pseudomonas fluorescens. Cubitto M.A., Ferrero M., et al. Oil-degrading ability of the immobilized bacterial consortium in cocopeat, rice hull powder and sodium alginate capsules was compared by Nunal et al. 2013;26(7):8291. Bioremediation of Crude Oil Contaminated Soil by Petroleum-Degrading Active Bacteria Jinlan Xu School of Environmental and Municipal Engineering, Xi an University of Architecture and Technology, Xi an, Shaanxi, China 1. Study on the Changes in Immobilized Petroleum-Degrading Bacteria Beads in a Continuous Bioreactor Related to Physicochemical Performance, Degradation Ability, and Microbial Community. 1Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran, 2Institute for Marine Environment Research (IAMC), CNR of Messine, Messina, Italy, Petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great Numerical simulation of bioremediation of poly aromatic. official website and that any information you provide is encrypted The poultry droppings had total heterotrophic bacterial and fungal counts of 4.2 104cfu/g and 1.8 104 . Int. Biodegradation of 2-chloroethanol by freely suspended and adsorbed immobilized. Federal government websites often end in .gov or .mil. Period. Bio-removal of diesel oil through a microbial consortium isolated from a polluted environment . The announcement was made by Gates Foundation CEO Mark, Hybrid Event to Live-Stream from University of Nairobi JOHANNESBURG, South Africa, 7 November, 2022-/African Media Agency(AMA)/- Africa.com, today announced that on November 17th, from 6:00 PM to 7:00 PM EAT (Nairobi), it will host Bill Gates, co-chair of the Bill & Melinda Gates Foundation, on its virtual platform in partnership with the University of Nairobi. Bioremediation of an Experimental Oil Spill in a Coastal Louisiana Salt Marsh. Omar S.H., Rehm H.J. Soil acidity decreased; soil organic matter, carbon content and exchangeable ions increased. Bioremediation is a technology that exploits the abilities of microorganisms and other natural habitat of the biosphere to improve environmental quality for all species, including man. Long-term soil pollution by hydrocarbons led to environmental deterioration through damaging the soil structure, altering plant growth and food chains, putting at risk animal, and human health. Turkey has recently been exhibiting an increasing development in terms of spreading oil pipe-line constructions. Carried out an experiment involving biostimulation with agricultural fertilizers to evaluate the biodegradation of hydrocarbon compounds found in a crude-oil-polluted agricultural soil at different levels of soil water. PurposeOil hydrocarbons are widespread pollutants in soil which pose serious threats to ecological environment. Many advances in biochemistry and molecular biology are now applied in various bioremediation efforts (Olson and Tsai, 1992).According to some investigators (Barbee, et al., 1996), bioremediation does not always result in complete mineralization of organic compounds. In a few months, Baku-Tiflis-Ceyhan pipe-line would be carrying 50 million ton crude oil per year from Ceyhan through all over the world. Petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. HHS Vulnerability Disclosure, Help (Bouwer,1992).Bioaugmentation and biostimulation are two approaches tobiore mediation geared toward enhancing and speeding up the process. higher oil reduction by the cocopeat-immobilized cells is presumably due to highly sustained microbial population attached to surface of the biocarrier, providing protective niche, the porous nature of cocopeat might allow efficient substrate diffusion, slow release of nutrients, and acceleration of oxygen transfer, thus providing a favorable niche for hydrocarbon utilization. This may be due to the fact that bioremediation techniques do not leave any negative effects on the soil. doi: 10.1016/j.marpolbul.2014.03.027. Four samples, each having crude oil to water ratio of 1:4 was used. Bioremediation of crude oil polluted seawater by a hydrocarbondegrading bacterial strain immobilized on chitin and chitosan flakes. The potential re-usability of kaolinite after the initial use for oil sorption was analysed and 10 g of burnt kaolinite sorbed 43.62 per cent, while 20 g sorbed 58.90 per cent. contaminated soil, groundwater, and sedi-ment is treated on-site. On the other hand, Inorganic supports generally have one major advantage over other materials, namely, their toughness and etc. 2022 Dec;33(6):621-639. doi: 10.1007/s10532-022-09999-6. [52] employed Pseudomonas aeruginosa UG14 encapsulatedin alginate, clay and skim milk for degradation of phenanthrene. Elimination of cell washout problems at high dilution rates. Radwan et al. -, Appl Environ Microbiol. Close. FOIA Epub 2016 Feb 11. Removal of the infected material, while costly, is also an option. The researchers have proved that biological methodology is versatile, high stability, broad applications in various areas, economical and efficient for the remediation ofpetroleum [6]. Groboillot A., Boadi D.K., Poncelet D., Neufeld R.J. Immobilization of cells for application in the food industry. The spill site of about 15.6 hectares was situated between latitude 4N and longitude 7 7.5E, in Eleme Local Government Area of Rivers state. ENVIRONMENTAL experts have warned that improper disposal of waste oil is a major cause of contamination of the ecosystem and chronic health conditions now seen in the public. Bioremediation of petroleum hydrocarbon-contaminated environment is a potentially important application of Environmental Biotechnology. Non hydrocarbon compounds may also be found in crude oil and they include porphyrins and their derivatives (Callot and Ocampo, 2000). Make a Mobile Transfer or POS Payment of 3,000 to any of the Account Below. Save my name, email, and website in this browser for the next time I comment. Bioconversion of waste materials to industrial products. Your email address will not be published. Bioaugmentation involves the addition of external microbial population (endogenous or exogenous) to the polluted site. Pollut. strings of text saved by a browser on the user's device. Metals that could be found in crude oil via their association with porphyrins include nickel, vanadium, iron, zinc, cobalt, titanium and copper (Chicarelli, et al., 1990.) Another study published in Research Journal of Applied Sciences employed RENA to remediate oil-contaminated site in the Gokana local government area of Rivers State of Nigeria between January and September 2006. 2010). Verma M., Brar S.K., Blais J.F., et al. although the end- for potential crude oil degrading microrganisms. Bookshelf [Obidi Olayide F, Onuoha Angus C, Nwachukw Simon C. Bioremediation of Crude Petroleum Polluted Stagnant Recently the immobilization of whole cells have been developed as biocatalysts in environmental pollutions when are used for multi enzyme systems. Bioremediation has become an attractive alternative to physicochemical methods of remediation of polluted sites. Immobilized cells are widely tested for a variety of applications. government site. After a cleanup exercise, detoxification of soils polluted with residual petroleum hydrocarbon compounds is necessary. 1998 Jul;64(7):2578-84 Accessibility The number of cells adhering to the support depend on the kind of support. Bioremediation is an attractive and successful cleaning technique to remove toxic waste from polluted environment. The microbial and physicochemical properties of the soil samples varied with the different plots and at different periods of remediation. For improved and sustainable performance of bioremediation, the system requires the development of cost-effective carrier substrates which undergo slow biodegradability and . Mar. Comparative evaluation of growth performances for cassava crop grown in crude-oil-polluted soils remediated using biodegradation technique (RENA) adopted by a reputable oil company in Nigeria and the innovative CNB-Tech. In the last few years, the application of biotechnological processes that involvesing microorganisms with the objective of solving environmental pollution problems, is rapidly gro-wing. Isolation and characterization of alkane degrading bacteria from petroleum reservoir waste water in Iran (Kerman and Tehran provenances). Bioremediation of petroleum contaminated environments is a process in which the biological pathways within microorganisms or plants are used to degrade or sequester toxic hydrocarbons, heavy metals, and other volatile organic compounds found within fossil fuels.Oil spills happen frequently at varying degrees along with all aspects of the petroleum supply chain, presenting a complex array of . All of the methods of immobilization such as Adsorption, Covalent Binding, Entrapment and Encapsulation were tried for bioremediation of crude oil. Organic carriers are such as modified celluloses, dextran, chitosan and agarose, and inorganic carriers are such as zeolite, clay, anthracite, porous glass, activated char-coal, and ceramics [21]. Magner E. Immobilisation of enzymes on mesoporous silicate materials. Due to low literacy level, oral interviews were conducted on the community representatives to elicit information on factors such as (i) type of actions taken during the RENA remediation project, (ii) common utility of the land area prior to spill and (iii) experiences of farmers utilizing the remediated land area. Aug 15, 20181. It is the optimization of natural biodegradation in which micro-organisms chemically alter and break down organic molecules into other substances such as carbon dioxide, fatty acids, and water, in order to obtain energy and nutrients. Int. Compliance analysis requires examination of the contaminated site in the light of the governing regulation and the action plan. Margaritis A., Merchant F.J. Advances in ethanol production using immobilized cell systems. 2006; 57:222-228. doi: 10.1016/j.ibiod . Many different forms of cell immobilization have been used including Adsorption, Covalent Binding, Entrapment and Encapsulation (Fig. In fact, there are more than ve The release of crude oil in the marine environment during exploitation and transportation cause serious environmental pollution, owing to the presence of toxic organic compounds. Scott C.H. Disadvantages of cells immobilized using the adsorption technique is a the very high rate of leakage from matrix due to weak interactions, unstable interactions, no possibility to control the loading, so the reproducibility is also low [27, 28]. Chen Y.M., Lin T.F., Huang C., Lin J.C., Hsieh F.M. Ohta T., Ogbonna J.C., Tanaka H., et al. 2015 Jul 31;9:48-54. doi: 10.2174/1874285801509010048. This adsorption system could control pollution and also be easilyy used and with low costs. -. Bioremediation 3 0 Engineering pollutant removing. This study revealed that it compares and has the potential to excel over some existing biodegradation procedures employed by many oil industries, especially in developing countries. Maximum reductions in value of 94.04 per cent, 97.45 per cent, and 99.09 per cent were achieved for turbidity, BOD, and THC respectively at the microbial consortium load of 1x 10 6cfu/ml.. F92 on polyurethane foam (PUF) in the bioremediation of petroleum hydrocarbons. Toggle navigation. By bioaugmentation, bio stimulation can be improved. official website and that any information you provide is encrypted Wiesel et al. government site. Khanpour-Alikelayeh E, Partovinia A, Talebi A, Kermanian H. Environ Monit Assess. [45]. Nikolopoulou M, Pasadakis N, Kalogerakis N. Mar Pollut Bull. Overmeyer C., Rehm H.J. The increasing of environmental pollution and the treating contaminated sites are necessary atat . Biofilms are surface-attached microbial communities consisting of multiple layers of cells embedded in hydrated matrices [17].