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A-level Chemistry This means that it will take less time to turn from blue to colourless, This experiment is not measuring the rate of dehydrogenase activity directly (through measuring the rate of substrate use or product made) but is instead predicting what the rate would be measuring the rate of electron / hydrogen release from the reactions of respiration, Distinguishing the end of the reaction and the colour change is subjective and therefore one person should be used to attempt to control this. "Types of Farming". TO CANCEL YOUR SUBSCRIPTION AND AVOID BEING CHARGED, YOU MUST CANCEL BEFORE THE END OF THE FREE TRIAL PERIOD. Biological Molecules / 2.1 Testing for Biological Molecules / 2.1.2 The Benedict's Test. Classification, Biodiversity & Conservation, 18.1.2 The Three Domains: Archaea, Bacteria & Eukarya, 18.2.4 Testing for Distribution & Abundance, 18.3.2 Reasons for Maintaining Biodiversity, 19.1.6 Genetic Engineering: Promoters & Marker Genes, 19.2 Genetic Technology Applied to Medicine, 19.3 Genetically Modified Organisms in Agriculture, 19.3.1 Genetically Modified Organisms in Agriculture, It is important that an excess of Benedicts solution is used so that there is more than enough copper (II) sulfate present to react with any sugar present, A positive test result is, therefore, a colour change somewhere along a colour scale from blue (no reducing sugar) to brown/brick-red (a high concentration of reducing sugar), A semi-quantitative test can be carried out by setting up standard solutions with known concentrations of reducing sugar (such as glucose), These solutions should be set up using a serial dilution of an existing stock solution, Each solution is then treated in the same way: add the same volume of Benedicts solution to each sample and heat in a water bath that has been boiled (ideally at the same temperature each time) for a set time (5 minutes or so) to allow colour changes to occur, It is important to ensure that an excess of Benedicts solution is used, Any colour change observed for each solution of a known concentration in that time can be attributed to the concentration of reducing sugar present in that solution, The same procedure is carried out on a sample with an unknown concentration of reducing sugar which is then compared to the stock solution colours to estimate the concentration of reducing sugar present, It is also possible to standardise this test but instead of waiting a fixed amount of time for a range of colours to be observed, time how long it takes for the first colour change to occur (blue to green), The higher the concentration of reducing sugar in a sample, the less time it would take for a colour change to be observed, To avoid issues with human interpretation of colour, a colourimeter could be used to measure the absorbance or transmission of light through the sugar solutions of known concentration to establish a range of values that an unknown sample can be compared against a calibration curve, Serial dilutions are created by taking a series of dilutions of a stock solution. 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The advantage for living organisms is that it: This is vital in maintaining temperatures that are optimal for, As blood passes through more active (warmer) regions of the body, heat energy is absorbed but the temperature remains fairly constant, Water in tissue fluid also plays an important regulatory role in maintaining a constant body temperature, In order to change state (from liquid to gas) a large amount of thermal energy must be absorbed by water to break the, This is an advantage for living organisms as only a little water is required to evaporate for the organism to lose a great amount of heat, Hydrogen bonds between water molecules allows for strong, This allows columns of water to move through the xylem of plants and through blood vessels in animals, This also enables surface tension where a body of water meets the air, these hydrogen bonds occur between the top layer of water molecules to create a sort of film on the body of water (this is what allows insects such as pond skaters to float), This also enables water to move up the xylem due to transpiration. glucose) will dissolve in it. 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